Title Epigenetics로 유전자 파헤치기(ChIP-Seq과 ATAC-Seq 서비스)
Writer Admin
Date 2024-02-07
Attached File

▶ 실험분석 서비스 문의 : 02-3141-0791, service@e-biogen.com
ChIP-Seq & ATAC-Seq INFO
ChIP-Seq(Chromatin Profiling) Service ATAC-Seq(Transposase-Accessible Chromatin Profiling) Service
- Sample Requirement : > 20 ul IP-DNA or Input DNA
- Library Method : NEB Ultra DNA Library kit
- NGS run format : NovaSeq 6000, PE100bp
- Data Yield : ~4G/sample
- Turnaround time :~3 weeks after Library QC
- DATA Analysis : ExPADA report, Peak Annotation, Motif Discovery, IGV, BedGraph 등
- Sample Requirement : ~1x106 cells in 1ml media(Cell viability >80%)
- Library Method : ACTIVE MOTIF® ATAC-Seq kit
- NGS run format : NovaSeq 6000, PE100bp
- Data Yield : ~ 6G/ sample
- Turnaround time :~5 weeks after cell QC
- DATA Analysis : ExPADA report, Peak Annotation, Motif Discovery, IGV, BedGraph 등

ExPADA for Epigenetics Data Analysis
ExPADA(Excel based Peak Annotation and Differential enrichment Analysis)는 연구자들이 DNA-Seq 데이터를 보다 쉽고 직관적으로 다룰 수 있도록 만든 엑셀 기반의 분석 도구입니다. Peak Annotations, Motif results, DA Analysis 등을 제공하고 있습니다.

 

  • Gene Ontology 분석
  • Cluster 별 Significant data analysis
  • Volcano Plot 작성 및 gene marking
  • Venn Diagram 분석 및 해당 영역 gene filtering
  • KEGG Mapper – Search&Color Pathway Support
  • MeV Clustering Heatmap Support
  • DAVID Functional Annotation Support
  • Selected genes 발현 패턴 그래프 작성
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