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SERVICE

DNA-Seq

Enzymatic Methyl-Seq (EM-Seq)

Enzymatic Methyl Sequencing (EM-Seq) is an enzyme-based approach that increases the reliability of methylation analysis by minimizing DNA damage caused by conventional bisulfite treatment methods. It effectively detects 5-mC and 5-hmC, provides various analysis results such as methylation calling, DMC & DMR (Differentially methylated cytosines and regions) along with ExPADA report.

Service Feature

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Sample requirement

>2µg gDNA

Library method

NEBNext® Enzymatic Methyl-seq kit

NGS run format

NovaSeq, PE150bp

Data yield

~120G/sample

Turnaround time

~6 weeks after DNA QC

Sample type

gDNA, cfDNA⁺

+ If you need gDNA preparation, you can proceed through consultation.

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Progress

  • 1

    DNA QC

  • 2

    Library prep.

  • 3

    NGS

  • 4

    Data Analysis

  • 5

    Report

Data Analysis

We support ExPADA report, various analysis such as Methylation calling, DMC and DMR ((Differentially methylated cytosines and regions), correlation, PCA plot.

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