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Typical applications for the oligos are for use in microarray experiments, dye terminator sequencing reactions, gene building, polymerase chain reactions (PCR), Ligase Chain Reaction (LCR), hybridization studies, site directed mutagenesis, cloning, RTPCR antisense studies, siRNA, dual labeled probes
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Production details |
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Scales of synthesis
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0.01 ¥ìM, 0.05 ¥ìM, 0.2 ¥ìM, 1 ¥ìM, 2 ¥ìM, 5 ¥ìM, 10 ¥ìM and 15 ¥ìM
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Purification methods
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Desalted (RP Cartridge), HPLC, Elchrom Submarine Electrophoresis, PAGE
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Quality Analysis
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Capillary Gel Electrophoresis, PAGE, Analytical HPLC, Pyrosequencing
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Available Modifications
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Standard modifications
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5¡¯
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3¡¯
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Internal
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Aminolink-C6 (TFA), Aminolink-C6 (MMT), Disulfide
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Aminolink-C3, Aminolink-C7
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Phosphorylation, Aminolink-C12
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Digoxigenin, Thiol C6, Biotin
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Dye
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Cy2, Cy3, Cy3.5, Cy5, Cy5.5, HEX, TET
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BH Quencher 1, BH Quencher 2, Dabcyl
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IR Dy681, IR Dy781, Dy547 (Cy3 alternative), Dy647
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(Cy5 alternative), Dy554, Texas Red, Rhodamine, CAL Fluor Orange 650
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JOE, ROX, Tamra, 6 FAM, Fluorescein
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Base Modification
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Bromo-deoxyUridin
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Uridine, Uracyl, Deoxyinosine
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Antisense
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PTO
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Molecular Beacons/ TaqMan probes
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FAM, HEX, TET, ROX, JOE, Cy Dyes, Texas Red, Others
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