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Almost all the Life Sciences Scientific community can expect seeing an extraordinary surge of information related to roles of epigenetic events in their respective research.
There is a more and more obvious coordinated interplay between histone modifications and histone variants, DNA methylation, RNA components, ATP-dependent chromatin remodelling, and histone-specific assembly factors that regulates gene expression.
There are tremendous opportunities offered by the use of immuno-techniques like immunoprecipitation assays
(especially in combination with DNA micro arrays and High Throughput sequencing technologies).
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Our Shearing methods provide you with easy and highly reproducible chromatin shearing protocols which are:
• The first key for a successful ChIP
• Fast, optimized and user-friendly standardised for better reproducibility and as a consequence, data analysis is made easier
With our ChIP Sonication Control kit™ : monitor the sonication efficiency looking at the quality of the sheared chromatin.
(cat. #: kch-sonctl-001)
With our Shearing-ChIP kit™ : prepare large amounts of sheared chromatin ready-to-ChIP. (cat. #: kch-shchro-40)
With our Shearing module : prepare sheared chromatin ready-to-ChIP, then ChIP with our kits: either the Red ChIP
traditional protocol or the OneDay rapid method. (cat. #: kch-redmod-100).
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Chromatin immunoprecipitation (ChIP). ChIP assay offers a huge potential to boost knowledge about the regulation of the
genome expression. Used in a large number of life science disciplines and addressing several essential questions like cellular
differentiation, tumour suppressor gene silencing,…
ChIP, ChIP-on-chip* and ChIP Seq* are used to investigate interactions between proteins and DNA in vivo. It allows
the identification of binding sites of DNA-binding proteins in a very efficient and scalable way for proteins generally operating in
the context of chromatin like transcription factors, replication-related proteins, histones, their variants, and histone modifications.
* ChIP-on-chip and ChIP-Seq can localize protein binding sites which may help in identifying functional elements in the
genome (whole-genome or specific genomic regions).
All our products have been extensively validated in ChIP on various targets. The combination of all our Quality Controlled kits, reagents and equipment is the perfect starting point to your success.
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Transcription
ChIP kit (red)
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Histone
ChIP kit(orange)
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OneDay ChIP kit
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LowCells magnetic
ChIP kit
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Optimized for
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Transcription factors and co-factors studies
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Histones & modified histones
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All DNA-protein interaction
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All DNA-protein interaction
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Ideal for
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Traditional use
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Traditional use
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Intensive use
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Intensive use / rare material
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Suitable for ChIP-on-chip
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yes
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yes
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yes
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yes
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Amount of cells/IP
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10e6
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10e5
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1.5-2 10e6
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10e3 - 10e4
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Time from cell collection to PCR
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3 days
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3 days
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1 or 2 days
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1 or 2 days
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Buffers & Reagents for
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Cell fixation - collection
- lysis / Shearing / IP
/ DNA purif
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Cell fixation - collection
- lysis / Shearing / IP
/ DNA purif
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IP / DNA purif
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Cell fixation - collection
- lysis / IP / DNA purif
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Control Antibodies
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anti-TBP or RNA Pol II(3) / anti IgG
(rabbit or mouse) (1)
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anti H3 (K4me3) /
antiIgG
(rabbit or mouse) (1)
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anti IgG
(rabbit or mouse) (1)
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Two + ctl Ab included (2)
/ anti IgG
(rabbit or mouse) (1)
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Control PCR primers pairs
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GAPDH / idem -0,5kb
/ idem -1kb / c-fos
/ beta actin
/ myoglobin exon 2
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BMX / c-fos / beta actin
/ myoglobin exon 2
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-
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SAT 2 / c-fos / beta actin
/ myoglobin exon 2
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Specific Key feature
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Traditional, lots of publications, comprehensive, ...
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Traditional, lots of publications, comprehensive, ...
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Quick, one buffer, adaptable to all kind of studies, no Phenol:Chloro, ...
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Quick, low amount of
material / IP, sensitive,
one buffer, adaptable to
all kind of studies, no
Phenol:Chloro,...
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#IP per kit
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18
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18
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60 or 180
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16
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(1) Rabbit anti IgG as standard, mouse delivered on request
(2) Two Ab to be chosen in the Diagenode catalogue
(3) RNA Pol II ctl Ab delivered on request
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During DNA replication, methylation is one of the post-synthesis modifications. It has been proven to be manifested in a
number of biological processes such as regulation of imprinted genes, X chromosome inactivation, tumour suppressor gene
silencing in cancerous cells and also acts as a protection mechanism adopted by pathogen DNA. Methylation usually occurs
in the CpG islands, a CG rich region, upstream of the promoter region.
Diagenode proposes two complementary methods for the study of DNA methylation patterns: Methylated DNA
Immunoprecipitation and Bisulfite conversion.
MeDIP kit™ : The Novel Diagenode Methyl kit is designed to immunoprecipitate methylated DNA (Methyl DNA IP).
This brand new Methyl DNA IP method provides you with methylated DNA (meDNA) and unmethylated DNA (unDNA) controls
to be used together with your DNA sample allowing direct correlation between IP’d material and methylation status.
This methylation analysis is highly specific and each IP is quality controlled: essential keys for reliable results.
The kit is:
• Specific and quantitative (several internal controls)
• Robust and simple (all in one tube)
• Fast (less than 3 days)
• User-friendly (comprehensive manual and quick start)
In addition, the MeDIP kit™ is validated for sample preparation prior to MeDIP-on-chip and MeDIP-seq assays.
One format: 10 IPs per kit.
(cat. #: mc-green-001)
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MethylEasy™ Methylation Detection Range
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| Diagenode promotes and distributes the kit range engineered by the inventors of the MethylEasy™ methodology used worldwide
for analysing chemical changes that occur in DNA in cancer, aging, stem cells and cell reprogramming.
MethylEasy™
• Xceed (cat # MEA-BISXCE-040): Rapid, ultra-sensitive, 40 reactions
• Original Bisulphite Kit (cat # MEA-BISLPH-025): Original precipitation based kit, 25 reactions
• High Throughput for Centrifuge (cat # MEA-BISWEC-096c): Original method, 96 well format for centrifuge,
any centrifuge that will accommodate a height of 5.1cm (2.0in)
• High Throughput for Vacuum (cat # MEA-BISWEC-096c): Original method, 96 well format for Vacuum Manifold.
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Original MethylEasy™
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MethylEasy™ Xceed
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Min 1ng of DNA
Denature DNA with NaOH and incubate for 15 minutes
Add conversion reagents and incubate for 4-16 hours
Precipitate and wash the DNA, 45 to 75 minutes
Resuspend the DNA and desulphonate for 30mins to 1hour
Full conversion 6 to 18 hours total time
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Min 50 pg of DNA
Idem
Add conversion reagents and incubate for 45 mins
Purify the DNA via column for 10 mins
Desulphonate for 20 mins
Full conversion 90 minutes total time
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Selection of our Kits
We achieve our selection of our kits through:
• Collaborators and customer suggestions (licensing)
• Research focus areas
• Conferences
We make our own antibodies and we source antibodies from other suppliers (academic laboratories, primary manufacturers).
Kits QC
We make every effort to offer our customers the best kits regardless of their source (90% of them are developed internally).
We struggle to optimize our kits regarding different conditions and targets. Our policy is to follow the most stringent testing conditions and to publish all information on our website.
Our customer support policy
Our team gives you the guarantee that:
• At any time, you will receive all support and available information on any of our product as including contact details of
other customers of the same kit.
• If, after optimization, they do not perform as described on the datasheet, we will replace or refund any faulty products if
reported within 120 days of purchase.
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